Biotechnology: Antibodies & Their Role In Therapeutics

Last updated 9/2022
MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHz
Language: English | Size: 2.02 GB | Duration: 5h 33m
Learn about Antibodies: Their Structure, Types, Functions and Role in Therapeutics & Diagnostics | Immunology | Biology
What you'll learn
1. Structure of antibodies
2. Types of light and heavy chains of antibodies
3. Complementarity Determining Regions (CDRs) & how they bind to antigen
4. Immunoglobulin domains
5. Framework region
6. Classes of antibodies: IgG, IgM, IgA, IgE, IgD & their biological activities
7. What is opsonization, ADCC and complement system
8. Pathways of complement activation
9. Regulation of complement system
10. Organization and expression of immunoglobulin genes
11. Mechanism of VJ and VDJ recombination
12. Generation of antibody diversity
13. Somatic Hypermutation
14. Class switching mechanism & factors governing it
15. Expression of membrane bound & secreted form of immunoglobulins
16. B cell receptors (BCRs)
17. Simultaneous expression of IgM and IgD on B cells
18. Regulation of Ig gene transcription
19. Differences between polyclonal and monoclonal antibodies
20. Hybridoma Technology
21. Therapeutic monoclonal antibodies
22. Antibody engineering: Chimeric monoclonal antibodies
23. Humanized monoclonal antibodies
24. Fully human monoclonal antibodies
25. Immunogenicity of engineered monoclonal antibodies
26. Immunotoxins
27. Bispecific antibodies
28. Abzymes
29. Convalescent plasma therapy
30. How is plasma therapy being used for the treatment of COVID-19 disease
Requirements
Knowledge of basic biology
Motivation to learn
Description
Immune system is the body's own army that fights against the invading pathogens and toxins. You can compare it to a nation's defense forces. The army, the air force and the navy all work for the nation's defense. Similarly, the immune system works for the body's defense. Everywhere around us, there are dangerous bacteria, viruses, parasites and toxins that can attack and harm our body. But our immune system defends us from these pathogens.Antibodies, also known as immunoglobulins, are one of the crucial warriors produced by our immune system to fight off these nasty invading pathogens. In absence of antibodies, these pathogens can attack our nerve cells, kidney cells, heart cells and other vital parts of the body.Once attacked by these nasty pathogens, the cells stop functioning, which in turn, may lead to serious illness. But when antibodies come into action, everything changes. They bind to the invading pathogens and prevent them from attacking our body cells.So what do these antibodies look like, what is their structure and how do they fight off such dangerous pathogens? If you are also curious about these concepts and want to learn the science behind it, then you have come to the right place.This course is an invaluable resource for medical students, doctors and students of disciplines like biotechnology, biology, immunology, genetics, molecular biology, cell biology and bioinformatics. In the course, the most challenging concepts are presented in a simple and palatable format using animations and graphics.This course will cover different types of antibodies circulating in the bloodstream & tissue fluids and their respective functions. Additionally this course will also cover the genetic mechanisms which allow our immune system to generate such a diverse pool of antibodies because of which our body is able to recognize and respond to a variety of antigens.Further in this course, the class switching mechanism, expression of membrane bound & secreted forms of antibodies and regulation of expression of immunoglobulin genes have also been discussed.Moreover, you will also get to know what is passive immunity and what is active immunity.We will also discuss the differences between polyclonal and monoclonal antibodies. In this course, the hybridoma technology has been discussed in detail and how it can be used to mass produce monoclonal antibodies? And how monoclonal antibodies can be used as therapeutics?Additionally this course will introduce you to various engineered therapeutic antibodies like chimeric monoclonal antibodies, humanised monoclonal antibodies and fully human monoclonal antibodies. In this course, you will get to know how the antibody structure can be leveraged to create immunotoxins, bispecific antibodies, abzymes etc for the treatment of cancer, arthritis, other viral, hormonal and autoimmune diseases.In this course we will discuss what is convalescent plasma therapy or passive antibody therapy and how it is being used for the treatment of COVID-19 disease, the current ongoing pandemic. Last but not the least, the course will also highlight the relevance of antibodies in therapeutics and diagnostics.With a 30 day return policy, there is nothing to lose. If you feel the course is not worth your money, you can return it and get your money back. Though, we assure you that you will not be disappointed by your wise decision of enrolling in this course.Who this course is for:The course is designed for medical students, doctors, researchers and students of biology, immunology, biotechnology, genetics, molecular biology, cell biology and bioinformatics disciplinesAny student who has immune system as a subject in their curriculumAnyone looking to study biology or immunology at college or university and wants quick, to the point knowledge about antibodiesNewly qualified teachers who need some great resources on antibodiesAnyone who wants to get an in-depth knowledge about antibodies and our immune system
Overview
Section 1: Introduction
Lecture 1 Introduction
Section 2: Structure of Antibody/Immunoglobulin
Lecture 2 Basic structure of immunoglobulins or antibodies
Lecture 3 Functional components of Antibodies
Lecture 4 Summary- Basic structure of antibodies
Lecture 5 Antibody sequencing to know different types of light and heavy chains of Abs
Lecture 6 Types of light chains
Lecture 7 Types of heavy chains
Section 3: Detailed structure of Antibody/Immunoglobulin
Lecture 8 Immunoglobulin Domains
Lecture 9 Complementarity determining regions (CDRs)
Lecture 10 Framework region
Lecture 11 Binding of antigen to CDRs
Lecture 12 Role of Hinge Region
Lecture 13 Summary- Detailed structure of antibodies
Section 4: Antibody classes and their biological activities
Lecture 14 Immunoglobulin G or IgG
Lecture 15 Immunoglobulin A or IgA
Lecture 16 Immunoglobulin M or IgM
Lecture 17 Immunoglobulin E or IgE
Lecture 18 Immunoglobulin D or IgD
Lecture 19 Summary- Antibody classes and their biological activities
Section 5: Complement system
Lecture 20 Introduction to complement system
Lecture 21 Nomenclature of complement proteins
Lecture 22 Classical pathway of complement activation
Lecture 23 Alternative pathway of complement activation
Lecture 24 Lectin Pathway of complement activation
Lecture 25 Membrane Attack Complex (MAC)
Lecture 26 Summary- Complement system
Lecture 27 Regulation of the complement system
Lecture 28 Other functions of complement proteins
Section 6: Organization of immunoglobulin genes
Lecture 29 Germline & somatic hypermutation Theory
Lecture 30 Tonegawa's Theory- Immunoglobulin genes rearrange
Lecture 31 Organization of Ig genes
Lecture 32 Organization of gene segments encoding light chains
Lecture 33 Organization of gene segments encoding heavy chains
Section 7: V(D)J Recombination
Lecture 34 VJ recombination in kappa light chain DNA
Lecture 35 VJ recombination in ƛ light chain DNA
Lecture 36 VDJ recombination in heavy chain DNA
Lecture 37 Mechanism of variable region DNA rearrangements
Section 8: Generation of antibody diversity
Lecture 38 Combinatorial VJ and VDJ joining
Lecture 39 Junctional Flexibility
Lecture 40 P-addition
Lecture 41 N-addition
Lecture 42 Somatic Hypermutation
Section 9: Expression of immunoglobulin genes
Lecture 43 Class switching
Lecture 44 Mechanism of class switching
Lecture 45 Factors that govern class switching
Section 10: Membrane bound and secreted Antibodies/Immunoglobulins
Lecture 46 Membrane bound immunoglobulins and BCRs
Lecture 47 Expression of membrane bound or secreted Immunoglobulin
Lecture 48 Simultaneous expression of IgM and IgD on mature B cells
Section 11: Regulation of Immunoglobulin gene transcription
Lecture 49 Regulatory sequences in DNA
Lecture 50 Allelic exclusion
Lecture 51 Mechanism of allelic exclusion
Section 12: Antigenic determinants/Epitopes on Immunoglobulins
Lecture 52 Isotypic determinants
Lecture 53 Role of anti-isotype antibodies in detection of viral diseases
Lecture 54 Allotypic determinants
Lecture 55 Idiotypic determinants
Lecture 56 Types of anti-ID antibodies
Lecture 57 Specific uses of anti-ID antibodies
Section 13: ‌Polyclonal‌ ‌‌&‌ ‌Monoclonal‌ ‌Antibodies
Lecture 58 Polyclonal Antibodies
Lecture 59 Monoclonal Antibodies
Lecture 60 Hybridoma Technology (Production of Monoclonal Antibodies)
Lecture 61 Selection of Hybridoma cells producing Monoclonal Antibodies
Section 14: Antibody Engineering
Lecture 62 Advantages of therapeutic monoclonal antibodies over conventional drugs
Lecture 63 Chimeric Monoclonal Antibodies
Lecture 64 Humanized Monoclonal Antibodies
Lecture 65 Fully Human Monoclonal Antibodies
Lecture 66 Isolation of monoclonal antibodies directly from humans
Lecture 67 Immunogenicity of engineered monoclonal antibodies
Section 15: Monoclonal Antibody Therapy
Lecture 68 Immunotoxins
Lecture 69 Recombinant Immunotoxins
Lecture 70 Humanised and Fully Human Immunotoxins
Lecture 71 Bispecific Antibodies or Heteroconjugates
Lecture 72 Abzymes
Section 16: Convalescent Plasma Therapy for the treatment of COVID-19 disease
Lecture 73 Structural proteins of SARS-CoV-2
Lecture 74 What is Plasma Therapy or Passive Immunization?
Lecture 75 How is plasma therapy done?
Lecture 76 Limitations of Plasma Therapy
Section 17: Monoclonal antibody Therapy for the treatment of COVID-19 disease
Lecture 77 Monoclonal antibodies and COVID-19
Lecture 78 Isolation of monoclonal antibodies from COVID-19 recovered patients
Lecture 79 How plasma therapy & monoclonal antibody therapy are different from vaccination?
Section 18: What's Next?
Lecture 80 What's next?
The course is designed for medical students, doctors, researchers and students of biology, immunology, biotechnology, genetics, molecular biology, cell biology and bioinformatics disciplines,Any student who has immune system as a subject in their curriculum,Anyone looking to study biology or immunology at college or university and wants quick, to-the-point knowledge about antibodies,Newly qualified teachers who need a good resource on antibodies,Anyone who wants to get an in-depth knowledge about antibodies and our immune system
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